Heat labile udg
WebThe purpose of this investigation is to ensure a heat-labile UDG, which makes the application of uracil-DNA glycosylase to PCR easy. To 0.5 kb uracil-DNA amplified by PCR, UDG of the present invention (A of FIG. 12) and UDG of E. coli (B of FIG. 12) were added and separately treated at 50° C. for varied periods of time (FIG. 12). WebHeat-labile Bacterial UDG酶对双链DNA的活性低于对单链DNA的活性，对小的U-DNA寡核苷酸和dUMP有活性，但对RNA或正常的不含dUTP的DNA没有活性。 Heat-labile Bacterial …
Heat labile udg
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Web23 dec. 2010 · These heat-labile characteristics enabled Psp HJ147 UDG to control carry-over contamination in direct PCR without loss of the PCR product. Psp HJ147 UDG's contaminant control in both direct PCR and indirect PCR exhibited superiority over the UDG of the marine psychrophilic bacterium strain BMTU 3346 and that of E. coli. WebAfter the heat-labile UDG enzyme (NEB, cat.m0280, USA) treatment of the U-labeled second-stranded DNAs, the ligated products were amplified with PCR by the following conditions: initial denaturation at 95℃ for 3 min; 8 cycles of denaturation at 98℃ for 15 sec, annealing at 60℃ for 15 sec, and extension at 72℃ for 30 sec; and then final ...
Web제품 설명. Uracil DNA Glycosylase (UNG)는 우라실을 포함하는 DNA에서 deoxyribose 와 우라실 (U) 염기 사이의 N-glycosylic bond를 가수분해하여 탈필리미딘 부위를 만드는 역할을 한다. 본 효소는 우라실을 포함한 single strand DNA와 … WebThe gene encoding a heat-labile uracil-DNA glycosylase (UDG) from a psychrophilic, gram-positive marine strain (BMTU3346) has been cloned, sequenced, and expressed in …
WebHeat-labile UDG. UDG (Uracil-DNA Glycosylase，尿嘧啶-DNA 糖基化酶)可催化水解含有dU 的DNA 单链或双链的尿嘧啶碱基和糖磷酸骨架的N- 糖苷键，释放游离尿嘧啶，由此产生的无 … WebThe gene encoding a heat-labile uracil-DNA glycosylase (UDG) from a psychrophilic, gram-positive marine strain (BMTU3346) has been cloned, sequenced, and expressed in Escherichia coli. The UDG is a cold-active enzyme with an apparent temperature optimum of 35°C and a half-life of 2 min at 40°C.
Web碧云天生产的Uracil-DNA Glycosylase (Heat-labile, Cod)，也称Heat-labile Cod UDG，即热敏型鳕鱼UDG，是来源于大西洋鳕鱼(Gadus morhua cod)，可催化含尿嘧啶的DNA链中的尿 …
http://cache1.bioon.com.cn/picture/20167/20167291190950.pdf milliman seattle officeWeb2 feb. 2015 · Uracil-DNA Glycosylase, heat-labile contains the equally named enzyme found in the marine bacterium BMTU 3346. Like the UNG from E. coli it hydrolyzes uracil … milliman seattle waWebHeat-labile Uracil-DNA Glycosylase (1 U/μl) 技术规格 单位定义 一个单位定义为在 37°C，60 分钟内完全降解 1 μg 纯化的单 链尿嘧啶 DNA（噬菌体 M13，大肠杆菌 CJ236dut-ung-中 生长）所需的热敏 UDG 酶的量。 酶储存液：20 mM Tris-HCl，pH 8.0（4°C）， 0.1 mM EDTA， 100 mM KCl，1 mM DTT，50%甘油，0.5%Nonidet，0.5% Tween-20。 在缺乏稳 … milliman scholarshipWebAn optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl₂, and dNTPs. Optional UDG system includes the Heat-Labile UDG and dUTP. Application. Viral RNA detection; Gene-expression analysis; Molecular diagnosis; Endpoint one-step RT-PCR from any RNA transcript milliman seattle phone numberWebSince UDG remains partially active following heat treatment at 95°C, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform. ... 1X UDG Reaction Buffer 20 mM Tris-HCl 1 mM DTT 1 mM EDTA (pH 8 @ 25°C) Storage Buffer ... milliman study on direct primary careWebタカラバイオのPCRキャリーオーバー防止製品で使用している Uracil DNA Glycosylase (UNG), heat-labile（製品コード 2820） は、50℃ 10分の熱処理により完全かつ不可逆的に失活する。. キャリーオーバーを確実に不活性化できる量のUNGを加えても、PCRサイクル … milliman medinsight health waste calculatorWebThe Uracil-DNA Glycosylase derived from Gadus morhua has all the attributes of the enzyme derived from E. coli with the added benefit of being heat-labile. It is completely and … milliman south africa