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WebJan 1, 2024 · Trophoblastic cell lines are established models used to examine human placenta physiology and disease. We performed concurrent cytogenetic analyses of six established and well-studied trophoblastic cell lines including JAR, BeWo, JEG-3, AC-1M59, HTR8/SVneo, and ACH-3P. All cell lines showed near triploid or tetraploid … WebTo cite this cell line use: BeWo (RRID:CVCL_0044) Comments. Characteristics: Cells were initially transplanted to the cheek pouch of a hamster and maintained by serial passage for 8 years prior to the establishment of the cell line. Doubling time: ~30 hours (DSMZ). Karyotypic information: Near triploid karyotype (PubMed= 33096371 ).
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WebDec 1, 2024 · The BeWo cell line is used to study placental function, since it forms syncytium and secretes hormones after treatment with cAMP or forskolin. In contrast, the JEG-3 cell line fails to undergo substantial fusion. Therefore, BeWo and JEG-3 cells were used to identify a set of genes responsible for trophoblast fusion. Cells were treated with ... WebFetal bovine serum (FBS) is used as a growth supplement for the in vitro cell culture of eukaryotic cells. Animal sera—both bovine and non-bovine sera—are used in cell culture applications with the most widely used being fetal bovine serum (FBS). FBS contains growth factors and very low levels of antibodies, allowing for versatility in many ...
WebBeWo Cell Line is from human; Sex: F; Tissue: placenta; Morphology: epithelial-like; Profile: Human placenta derived cell line. WebGeneral information. Description. BeWo cells are a human placental cell line that originated from a choriocarcinoma, which is a type of cancer that starts in the cells of the placenta. …
WebJun 23, 2024 · Moreover, BeWo cells show high glycolytic activity and so the culture medium has to be changed every 24-48 hours in order to prevent glucose exhaustion. Subculture the cells when they are 70-80 ... WebBuy from Supplier : Structured Review. ATCC human choriocarcinoma cell line bewo ... The BeWo cell line, which secrets high levels of β-HCG, was used as a positive control. Magnification, ×40. (B) Reverse transcription-polymerase chain reaction was used to determine the messenger RNA levels of CGB5 in the stably transfected cells. (C) The ...
WebFusion of the trophoblast-derived choriocarcinoma cell line BeWo can be triggered by forskolin. BeWo cells are regularly used as a cell culture model to mimic in vivo syncytialisation of placental villous trophoblast. The β …
WebOct 6, 2005 · In BeWo cells. Culture of BeWo cells over time resulted in almost two-fold increase in hCG secretion (Fig. 2A and B). Addition of Fk significantly raised hCG levels 2.6 and 3.7 fold at 48 and 72h, respectively, compared with control conditions (P < 0.01). TNFα alone had no effect on hCG secretion, while the combination of Fk/TNFα had a less ... crcam provence cote d\\u0027azurWebFeb 24, 2024 · BeWo cells were seeded in a black, clear bottom 96-well microplate at a cell density of approximately 3000 cells per well and supplemented with EGF (50 ng/mL), FSK (50 μM), rotenone (10 nM), and/or NAC (5 mM). The protocol was carried out as per the manufacturer’s instructions (Abcam DCFDA Cellular ROS Detection Assay Kit, … crcam nord midi-pyreneesWebData are presented as mean ± SD of at least 3 independent experiments (n = 3 – 4; in triplicate). a Comparison of β- hCG secretion of BeWo b30 cells upon 48 h treatment … makie definitionWebOct 8, 2024 · A & Z Express, Tucker, Georgia. 913 likes. Wholesalers & distributors for Hot Selling Products for Convenience Stores & Smoke Shops crcam provence alpes cote d\\u0027azurWebMay 6, 2024 · Syncytialization of BeWo cells was induced with forskolin, and fusion index was measured using a simple live cell imaging method . Briefly, BeWo cells were seeded on poly-l-lysine (Sigma, catalog no. P2636)–coated no. 0 cover glass for 24 hours at 5% CO 2 at 37°C. After 24 hours, BeWo cells were treated with 30 μM forskolin (Cell Signaling ... crc appellate appendixWebCCL-2 ™. HeLa cells are the first immortal human cells to be grown in culture and the basis for countless significant scientific discoveries. They were isolated in 1951 from a cervical carcinoma derived from a 31-year-old patient. Product category. Human cells. crca picardie en ligneWebNov 29, 2024 · RNA extraction and microarray hybridisation. For genomic profiling, stable green fluorescent protein (GFP) and Discosoma red fluorescent protein (dsRed) expressing BeWo and JEG-3 cells were used in the fusion assay as described previously (Vatish et al., 2012).Briefly, BeWo and JEG-3 cells were treated with forskolin to induce fusion and the … crca le village montpellier